Characterization and comparison of the bacterial microbiota of Lysiphlebia japonica parasitioid wasps and their aphid host Aphis gosypii
Xueke Gao, Ruichang Niu, Xiangzhen Zhu, Li Wang, Jichao Ji, Lin Niu, Changcai Wu, Shuai Zhang, Junyu Luo, Jinjie Cui
近日，中国农业科学院棉花研究所虫害防控与生物安全创新团队发现，寄生蜂可能通过共享或改变寄主共生菌来适应和调节寄主环境。该研究对揭示共生菌-寄生蜂-寄主昆虫的协同进化，制定棉蚜新型防控策略和开展生物防治具有重大意义。相关研究结果发表于《害虫管理科学》。
据团队首席研究员崔金杰介绍，棉蚜是一种广泛分布于世界各国的常见害虫，会导致棉花叶片变黄、皱缩、生长缓慢，以至植株死亡。近年来，由于大量使用农药等因素，棉蚜自身抗性越来越高，抗性背景复杂。
寄生蜂是华北棉田棉蚜的重要寄生性天敌，田间寄生率可达到80%。针对棉蚜危害日益严重、爆发成灾、抗性增强等现状，研究寄生蜂如何影响棉蚜微生物群落的变化及其相互关系，对防治棉蚜具有重要意义。
该研究通过分析寄生蜂棉蚜茧蜂的共生菌组成和结构，以及寄主棉蚜被寄生不同时段共生菌组成和丰度变化，系统报道了寄生蜂对棉蚜共生菌组成和结构的影响。
研究结果表明，共生微生物可能是棉蚜与棉蚜茧蜂之间复杂的、多层次的代谢产物和营养物质网络共同进化的潜在驱动力。
该研究结果对揭示寄生蜂与寄主昆虫的协同进化，开发新型棉蚜防控技术等具有重要的理论和实践价值。
该研究得到了国家自然科学基金和中国农业科学院科技创新工程资助。
来源：中国科学报 李晨 梁冰
Abstract
BACKGROUND
Endosymbiotic bacteria have been reported to mediate interactions between parasitoids and their insect hosts. How parasitic wasps influence changes in host microbial communities and the relationship between them are of great importance to the study of host‐parasitoid co‐evolutionary and ecological interactions. However, these interactions remain largely unreported for interactions between Aphis gossypii and Lysiphlebia japonica.
RESULTS
In this study, we characterize the bacterial microbiota of L. japonica wasps at different developmental stages and monitor changes over time in the bacterial microbiota of their parasitized and nonparasitized aphid hosts, using metagenomic analysis of 16S rDNA sequencing data. Proteobacteria, Firmicutes, and Actinobacteria were the three most abundant bacterial phyla identified in L. japonica. We found that parasitism was associated with an increased abundance of Buchnera nutritional endosymbionts, but decreased abundance of Acinetobacter, Arsenophonus, Candidatus_Hamiltonella, and Pseudomonas facultative symbionts in aphid hosts. Functional analysis of enriched pathways of parasitized aphids showed significant differences in the ‘transport and metabolism of carbohydrates’ and ‘amino acid, lipid, and coenzyme biosynthesis’ pathways. Notably, the composition of symbiotic bacteria in wasp larvae was highly similar to that of their aphid hosts, especially the high abundance of Buchnera.
CONCLUSION
The results provide a conceptual framework for L. japonica interactions with A. gossypii in which the exchange of symbiotic microbes provides a means by which microbiota can potentially serve as evolutionary drivers of complex, multilevel interactions underlying the ecology and co‐evolution of these hosts and parasites.
位访客